Mercoledì 6 febbraio nono appuntamento del Ciclo di Seminari del DSF 2018/19 "LIPOtoxicity-mediated disruption of adult hypothalamic NEUral progenitor cells as a potential contributor in Metabolic Syndrome (LipoNEUMetS)".
L'evento si svolgerà dalle 13:00 alle 13:40 in aula seminari al II piano di l.go Donegani 2 (ex Wild).
Il talk sarà tenuto da Heather Bondi (UNIUPO – DSF).
Metabolic syndrome (MetS) represents a risk factor for the onset of type 2 diabetes (T2D) and other chronic diseases, including neurodegenerative disorders. The hypothalamus (Hyt) is the brain region that plays a key role in regulation of food intake and energy expenditure. In mice, high-fat diet (HFD) induces an increase of circulating and brain free fatty acids (FFA), which, in turn, can exert toxic effects (lipotoxicity) and potentially compromise Hyt functions. Adult neurogenesis (aNG) is a key form of neuroplasticity which also occurs in Hyt (aHytNG) and is impaired in mice exposed to HFD. This observation has generated the hypothesis that lack of remodelling in the Hyt network as a consequence of disrupted NG may potentially contribute to MetS. Little is currently known about the direct effects of lipotoxicity on aHytNG. The present project aims at understanding the in vitro effects of a FFA-enriched microenvironment on adult Hyt neural progenitor cells (aHytNPC), using an innovative and highly throughput image-based approach, namely High Content Analysis (HCA). We will use Palmitic acid (PA) to reproduce lipotoxicity and its potential underlying cellular and molecular effects on aHytNPC. Moreover, we want: i) to assess whether aHytNPC impairment by PA may be pharmacologically counteractable; ii) to identify novel drugs with protective properties against lipotoxicity. Our results may shed light on novel pathophysiological mechanisms that link MetS and brain dysfunction.